Thyroid-stimulating Hormone Binding to Beef Thyroid Membranes

Thyroid-stimulating Hormone Binding to Beef Thyroid Membranes

The effect of sugars on ‘*“I-thyroid-stimulating hormone binding to beef thyroid membranes was studied to determine their role in thyroid-stimulating hormone (TSH) binding. At 0.1 M concentration, N-acetylneuraminic acid produced a 3to 7-fold increase in TSH binding, was the only sugar to enhance TSH binding, and did so whether binding was determined in the cyclase medium or under conditions of...

Thyroid-stimulating Hormone Binding to Beef Thyroid Membranes RELATIOK TO ADENYLATE CYCLASE ACTIVITY

Binding of [3H]thyroid-stimulating hormone (TSH) and iz51-TSH to beef thyroid membranes was studied under conditions to give optimum binding. Binding was maximal at pH 5.5, was complete within 5 min, and was stable for at least 90 min at 0 and 22” for low and high TSH concentrations, respectively. At pH 7.5, two binding sites were found with apparent affinity constants of 2.2 to 3.1 X lo8 M-I a...

Thyroid-stimulating hormone binding to beef thyroid membranes. Relation to adenylate cyclase activity.

Binding of [3H]thyroid-stimulating hormone (TSH) and iz51-TSH to beef thyroid membranes was studied under conditions to give optimum binding. Binding was maximal at pH 5.5, was complete within 5 min, and was stable for at least 90 min at 0 and 22” for low and high TSH concentrations, respectively. At pH 7.5, two binding sites were found with apparent affinity constants of 2.2 to 3.1 X lo8 M-I a...

Thyroid-stimulating hormone binding to beef thyroid membranes. Role of N-acetylneuraminic acid.

The effect of sugars on 125I-thyroid-stimulating hormone binding to beef thyroid membranes was studied to determine their role in thyroid-stimulating hormone (TSH) binding. At 0.1 M concentration, N-acetylneuraminic acid produced a 3- to 7-fold increase in TSH binding, was the only sugar to enhance TSH binding, and did so whether binding was determined in the cyclase medium or under conditions ...

Thyroid Stimulating Hormone Thyroid Stimulating Hormone